Granuloma Simulations

an on-line supplement for

Microenvironments in Tuberculous Granulomas Are Delineated by Distinct Populations of Macrophage Subsets and Expression of Nitric Oxide Synthase and Arginase Isoforms

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Joshua T. Mattila, Olabisi O. Ojo, Diane Kepka-Lenhart, Simeone Marino, Jin Hee Kim, Seok Yong Eum, Laura E. Via, Clifton E. Barry III, Edwin Klein, Denise E. Kirschner, Sidney M. Morris, Jr., Philana Ling Lin, JoAnne L. Flynn, Microenvironments in Tuberculous Granulomas Are Delineated by Distinct Populations of Macrophage Subsets and Expression of Nitric Oxide Synthase and Arginase Isoforms, J Immunol 2013 191:773-784; published ahead of print June 7, 2013, DOI: 10.4049/jimmunol.1300113, PMID: 23749634, PMCID: 3746594




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Supplemental Data 2
Simulation of M1/M2 macrophage dynamics in a granuloma over 200 days


This video shows M1 (green)-M2 (red) dynamics of the granuloma in Figure 9A of the main text. It is a caseous granuloma, with 2x105 CFU at day 200 post infection (mostly extracellular and replicating in the middle [brown]), average ratio (M1-M2) ~0.25 at day 200. The threshold to assign macrophages to either M1 or M2 is 0.5 (if threshold > 0.5, the macrophage is M1). The ~Sx~T represents a necrotic spot, where no cell is alive and the extracellular bacteria (if any) are dormant. The grey squares represent vascular sources (entry points for macrophages and T cells).

Right click or CTRL-click on AVI icon, select "Save Link As" or "Save Target As" to download the archived AVI movie file version of time-lapse simulations of controlled infection

To extract the AVI movie from the archive, download the archive to your Mac or Windows desktop, double-click on them, and follow the on-screen instructions.

To extract on Linux, Unix, BSD, etc... use the command in a shell 'unzip (filename.zip)' or a desktop extraction utility

Flash time-lapse simulations are best played using Flash version 8 or greater



Search engine friendly content

To preview the movie, click on the graphic image above


Supplemental Data 3
Simulation of M1/M2 macrophage dynamics in a granuloma over 200 days


This video shows M1 (green)-M2 (red) dynamics of the granuloma in Figure 9C of the main text. It is a small caseous granuloma, with 200 CFU at day 200 post infection (both intracellular and extracellular in the center of the granuloma), average ratio (M1/M2) ~0.37 at day 200. The threshold to assign macrophages to either M1 or M2 is 0.5 (if threshold > 0.5, the macrophage is M1). The grey squares represent vascular sources (entry points for macrophages and T cells).

Right click or CTRL-click on AVI icon, select "Save Link As" or "Save Target As" to download the archived AVI movie file version of time-lapse simulations of TNF Deletion

To extract the AVI movie from the archive, download the archive to your Mac or Windows desktop, double-click on them, and follow the on-screen instructions.

To extract on Linux, Unix, BSD, etc... use the command in a shell 'unzip (filename.zip)' or a desktop extraction utility

Flash time-lapse simulations are best played using Flash version 8 or greater



[ Time-Lapse Simulations Homepage | Kirschner Lab Homepage ]