Microenvironments in Tuberculous Granulomas Are Delineated
by Distinct Populations of Macrophage Subsets and
Expression of Nitric Oxide Synthase and Arginase Isoforms
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Supplemental Data 2
Simulation of M1/M2 macrophage dynamics in a granuloma over 200 days
This video shows M1 (green)-M2 (red) dynamics of the
granuloma in Figure 9A of the main text. It is a caseous granuloma, with
2x105 CFU at day 200 post infection (mostly extracellular and replicating in
the middle [brown]), average ratio (M1-M2) ~0.25 at day 200. The threshold
to assign macrophages to either M1 or M2 is 0.5 (if threshold > 0.5, the
macrophage is M1). The ~Sx~T represents a necrotic spot, where no cell is
alive and the extracellular bacteria (if any) are dormant. The grey squares represent vascular sources (entry points for macrophages
and T cells).
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select "Save Link As" or "Save Target As"
to download the archived AVI movie file version of
time-lapse simulations of controlled infection
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To preview the movie, click on the graphic image above
Supplemental Data 3 Simulation of M1/M2 macrophage dynamics in a granuloma
over 200 days
This video shows M1 (green)-M2 (red) dynamics of the
granuloma in Figure 9C of the main text. It is a small caseous granuloma,
with 200 CFU at day 200 post infection (both intracellular and extracellular
in the center of the granuloma), average ratio (M1/M2)
~0.37 at day 200. The threshold to assign macrophages to either M1 or M2
is 0.5 (if threshold > 0.5, the macrophage is M1). The grey squares represent vascular sources (entry points for macrophages
and T cells).
Right click or CTRL-click on AVI icon,
select "Save Link As" or "Save Target As"
to download the archived AVI movie file version of
time-lapse simulations of TNF Deletion
To extract the AVI movie from the archive, download the archive to your Mac or Windows desktop,
double-click on them, and follow the on-screen instructions.
To extract on Linux, Unix, BSD, etc... use the command in a shell 'unzip (filename.zip)' or a desktop extraction utility
